Basic blue 12 85% (dye content), Sigma-Aldrich®

Nile blue chloride has been used to stain non-aqueous phase in fluorescence microscopy studies.

Nile blue chloride is a green colored powder and is a member of oxazine group of dyes. It changes its color from blue (pH 9,4) to purple-red (11,0). Nile blue chloride has been used to detect DNA and lipids. In addition, it also helps to identify bacterial growth. Nile blue chloride also facilitates diagnosis of disease linked with amyloid accumulation.