3,3',5,5'-Tetramethylbenzidine (TMB) membrane peroxidase substrate

In the presence of peroxidases, TMB substrates can act as an electron donor for the conversion of peroxides to water.

• Reaction can be halted with acid to change the TMB to yellow
• Soluble blue end product read at 370 nm or 655 nm
• Incubation time varies depending on the assay conditions

TMB ELISA peroxidase substrate (3, 3', 5, 5'-tetramethylbenzidine) is a chromogenic substrate used for antibody reactivity in ELISA experiments.

TMB ELISA peroxidase substrate is designed for use in immunology and cancer research.

Delivery information: Supplied as a ready to use clear to very light blue colour liquid.