3,3',5,5'-Tetramethylbenzidine dihydrochloride hydrate

TMB is an excellent colorimetric substrate for detection of horseradish peroxidase labelled probes and is used with peroxidase and peroxidase coupled systems, particularly in ELISA techniques. It produces a soluble end product, which is blue in color and can be read spectrophotometrically. TMB has also been used in the quantitative determination of hemoglobin and in cytochemical staining for peroxidase. TMB is a hepatitis B antigen.

• Peroxidase Substrate: Sensitive detection of HRP activity in colorimetric assays
• Stable Salt Form: Dihydrochloride hydrate enhances solubility and shelf-life
• Clear Colorimetric Signal: Produces an intense blue color that can be quantified at 650 nm; turns yellow upon acid stop (read at 450 nm)
• High Sensitivity: Suitable for low-concentration analyte detection
• Broad Assay Use: Common in ELISA, blotting, and enzyme kinetics applications