Bis-trispropane ≥99% buffer

Buffer that is useful in the pH 6,3 to 9,5 range. This wide buffering range is due to its two pKa values, 6,8 (pKa1) and 9,0 (pKa2), being so close. Because of the wide buffering range, particularly down to pH 6 to 7, this buffer has been used to enhance the stability or activity of restriction enzymes, compared to Tris buffer (which is a poor buffer below pH 7.5 and has a comparatively large change in pKa with temperature).

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