The SPINeasy® RNA Kit for Feces delivers high yields of pure RNA without detectable DNA contamination in less than 20 min, making it particularly suitable for downstream applications, such as qPCR and RT-qPCR without the need of further inhibitor/DNA removal steps.

• For genomic, molecular biology, PCR, qPCR

  Personal protective equipment: safety goggles/faceshield, gloves

The composition of fecal samples is largely dependent on diet which includes fibers, undigested particles, bilirubin, complex polysaccharides, and lipids. Those compounds impair the sample homogenization, decrease both the quantity and quality of the extracted DNA. The SPINeasy® RNA Kit for Feces integrates our 1) state of art bead beating technology, 2) a lysis chemistry compatible with the widest range of samples and 3) our proprietary inhibitor removal expertise. A new technology of selective binding of RNA along with our FastDNAse I treatment allows the complete removal of DNA within 1-2 minutes, further reducing the processing time.

Components: Equilibration buffer (12 ml), lysing matrix yb (50 ea), phenol: chloroform: isoamyl alcohol (6 ml), buffer FR1 (40 ml), buffer FR2 (15 ml), buffer FR3 (48 ml), buffer FR4 (72 ml), buffer FR5 (36 ml), RNase-free water (10 ml), FastDNase enzyme (0,24 mL), FastDNase buffer (2 ml), RNA column (50 ea), 2 ml tubes (100 ea), collection tubes (150 ea), elution tubes (50 ea).

The SPINeasy® RNA Kit for Feces delivers high yields of pure RNA without detectable DNA contamination in less than 20 min, making it particularly suitable for downstream applications, such as qPCR and RT-qPCR without the need of further inhibitor/DNA removal steps.