Mouse eNOS ELISA Kit
About this item
Mouse eNOS ELISA Kit is a sandwich Enzyme-Linked Immunosorbent Assay (sELISA) designed for the in vitro quantitative determination of mouse eNOS in serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids.
Mouse eNOS ELISA Kit employs the sandwich enzyme immunoassay technique for the quantitative measurement of mouse eNOS in serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids. An antibody specific for eNOS has been pre-coated onto a 96-well microtiter plate. The standards and test samples are added into the wells and the eNOS present in each sample is bound to the wells by the immobilised antibody. Following incubation, the wells are washed and then incubated with Biotinylated Anti-eNOS Antibody, which binds the captured eNOS present in each well. Following incubation, unbound biotinylated detection antibody is removed by washing, and an HRP-Avidin conjugate is added to the wells and the microtiter plate is incubated. Following incubation and washing, TMB substrate solution is then used to visualise the HRP enzymatic reaction by catalysis to produce a blue-coloured product that changes to yellow after addition of acidic stop solution. The density of yellow is proportional to the amount of eNOS captured in each well. The concentration of eNOS can then be calculated by reading the O.D. absorbance at 450 nm in a microplate reader and referring to the standard curve.
Caution: This product is for Research Use Only. It is not for use in diagnostic or therapeutic procedures.
2 Options Available Below
Product Details & Documents
Mouse eNOS ELISA Kit is a sandwich Enzyme-Linked Immunosorbent Assay (sELISA) designed for the in vitro quantitative determination of mouse eNOS in serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids.
Mouse eNOS ELISA Kit employs the sandwich enzyme immunoassay technique for the quantitative measurement of mouse eNOS in serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids. An antibody specific for eNOS has been pre-coated onto a 96-well microtiter plate. The standards and test samples are added into the wells and the eNOS present in each sample is bound to the wells by the immobilised antibody. Following incubation, the wells are washed and then incubated with Biotinylated Anti-eNOS Antibody, which binds the captured eNOS present in each well. Following incubation, unbound biotinylated detection antibody is removed by washing, and an HRP-Avidin conjugate is added to the wells and the microtiter plate is incubated. Following incubation and washing, TMB substrate solution is then used to visualise the HRP enzymatic reaction by catalysis to produce a blue-coloured product that changes to yellow after addition of acidic stop solution. The density of yellow is proportional to the amount of eNOS captured in each well. The concentration of eNOS can then be calculated by reading the O.D. absorbance at 450 nm in a microplate reader and referring to the standard curve.
Caution: This product is for Research Use Only. It is not for use in diagnostic or therapeutic procedures.
Specifications for Product Family
Specifications
Specifications
- Catalog No:
- ANTIA1813-48
- ANTIA1813-96
- Target protein:
- eNOS
- eNOS
- Primary antibody reactivity:
- Mouse
- Mouse
- Assay Type:
- Sandwich
- Sandwich
- Format:
- Pre-coated
- Pre-coated
- Assay duration:
- Multiple steps
- Multiple steps
- Sensitivity:
- 0,67 ng/ml
- 0,67 ng/ml
- Detection range:
- 1,56 - 100 ng/ml
- 1,56 - 100 ng/ml
- Detection method:
- Colorimetric
- Colorimetric
- Detection limit:
- Intra-assay: CV <10%, Inter-assay: CV <12%
- Intra-assay: CV <10%, Inter-assay: CV <12%
- Sample type:
- Serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids
- Serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids
- Time to Results:
- 4 h 30 min
- 4 h 30 min
- Regulatory status:
- RUO
- RUO
- Storage temperature:
- 4 °C
- 4 °C
- Shelf life:
- Store for 6 months at 4 °C
- Store for 6 months at 4 °C
Specifications
Product Family Options
Product Information
- AvailabilityPrice
- ANTIA1813-48Mouse eNOS ELISA KitSpecifications:New Product
More
Specifications
Cat. No.ANTIA1813-48Target proteineNOSPrimary antibody reactivityMouseAssay TypeSandwichFormatPre-coatedAssay durationMultiple stepsSensitivity0,67 ng/mlDetection range1,56 - 100 ng/mlDetection methodColorimetricDetection limitIntra-assay: CV <10%, Inter-assay: CV <12%Sample typeSerum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluidsTime to Results4 h 30 minRegulatory statusRUOStorage temperature4 °CShelf lifeStore for 6 months at 4 °CNew Product - ANTIA1813-96Mouse eNOS ELISA KitSpecifications:New Product
More
Specifications
Cat. No.ANTIA1813-96Target proteineNOSPrimary antibody reactivityMouseAssay TypeSandwichFormatPre-coatedAssay durationMultiple stepsSensitivity0,67 ng/mlDetection range1,56 - 100 ng/mlDetection methodColorimetricDetection limitIntra-assay: CV <10%, Inter-assay: CV <12%Sample typeSerum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluidsTime to Results4 h 30 minRegulatory statusRUOStorage temperature4 °CShelf lifeStore for 6 months at 4 °CNew Product