Specifications
- Assay duration:Multiple steps
- Assay Type:Sandwich
- Format:Pre-coated
- Primary antibody reactivity:Rat
- Target protein:GAS 6
- Description:Rat GAS 6 ELISA kit
- Sample type:Serum, plasma, tissue homogenates, and other biological fluids
- Cross reactivity:Rat GAS 6 ELISA Kit exhibits high specificity and excellent specificity for the detection of rat GAS 6. No significant cross-reactivity or interference between GAS 6 and analogues was observed.
- Detection method:Colorimetric
- Time to Results:4 hours
- Shelf life:Store for 6 months at 4 °C
- Detection range:78,125 - 5000 pg/ml
- Storage temperature:4 °C
- Sample volume:100 μl
- Sensitivity:46,875 pg/ml
- Regulatory status:RUO
- Tests per kit:96 tests
Specifications
About this item
Rat GAS 6 ELISA Kit is a sandwich Enzyme-Linked Immunosorbent Assay (sELISA) designed for the in vitro quantitative determination of rat GAS 6 in serum, plasma, tissue homogenates, and other biological fluids.
- Ready-to-use ELISA kit
- Assay Precision: Intra-Assay: CV <8%, Inter-Assay: CV <10%
Rat GAS 6 ELISA Kit employs the sandwich enzyme immunoassay technique for the quantitative measurement of rat GAS 6 in serum, plasma, tissue homogenates, and other biological fluids. An antibody specific for GAS 6 has been pre-coated onto a 96-well microtiter plate. The standards and test samples are added into the wells and the GAS 6 present in each sample is bound to the wells by the immobilised antibody. Following incubation, the wells are washed and then incubated with Biotinylated Anti-GAS 6 Antibody, which binds the captured GAS 6 present in each well. Following incubation, unbound biotinylated detection antibody is removed by washing, and an HRP-Streptavidin conjugate is added to the wells and the microtiter plate is incubated. Following incubation and washing, TMB substrate solution is then used to visualise the HRP enzymatic reaction by catalysis to produce a blue-coloured product that changes to yellow after addition of acidic stop solution. The density of yellow is proportional to the amount of GAS 6 captured in each well. The concentration of GAS 6 can then be calculated by reading the O.D. absorbance at 450 nm in a microplate reader and referring to the standard curve.