- Assay duration:Multiple steps
- Assay Type:Sandwich
- Format:Pre-coated
- Primary antibody reactivity:Other
- Target protein:TIM 3
- Description:Porcine TIM 3 ELISA kit
- Sample type:Serum, plasma, tissue homogenates, and other biological fluids
- Cross reactivity:Porcine TIM 3 ELISA Kit exhibits high specificity and excellent specificity for the detection of porcine TIM 3. No significant cross-reactivity or interference between TIM 3 and analogues was observed.
- Detection method:Colorimetric
- Time to Results:4 hours
- Shelf life:Store for 6 months at 4 °C
- Detection range:0,156 - 10 ng/ml
- Storage temperature:4 °C
- Sample volume:100 μl
- Sensitivity:0,094 ng/ml
- Regulatory status:RUO
- Tests per kit:96 tests
Porcine TIM 3 ELISA kit is a sandwich Enzyme-Linked Immunosorbent Assay (sELISA) designed for the in vitro quantitative determination of porcine TIM 3 in serum, plasma, tissue homogenates, and other biological fluids.
- Ready-To-Use ELISA kit
- Assay Precision: Intra-Assay: CV <8%, Inter-Assay: CV <10%
Porcine TIM 3 ELISA kit employs the sandwich enzyme immunoassay technique for the quantitative measurement of porcine TIM 3 in serum, plasma, tissue homogenates, and other biological fluids. An antibody specific for TIM 3 has been pre-coated onto a 96-well microtiter plate. The standards and test samples are added into the wells and the TIM 3 present in each sample is bound to the wells by the immobilised antibody. Following incubation, the wells are washed and then incubated with Biotinylated Anti-TIM 3 Antibody, which binds the captured TIM 3 present in each well. Following incubation, unbound biotinylated detection antibody is removed by washing, and an HRP-Streptavidin conjugate is added to the wells and the microtiter plate is incubated. Following incubation and washing, TMB substrate solution is then used to visualise the HRP enzymatic reaction by catalysis to produce a blue-coloured product that changes to yellow after addition of acidic stop solution. The density of yellow is proportional to the amount of TIM 3 captured in each well. The concentration of TIM 3 can then be calculated by reading the O.D. absorbance at 450 nm in a microplate reader and referring to the standard curve.