- Assay duration:Multiple steps
- Assay Type:Competitive
- Format:Pre-coated
- Primary antibody reactivity:Other
- Target protein:Prostaglandin E1
- Description:Prostaglandin E1 ELISA kit
- Sample type:Serum, plasma, tissue homogenates, and other biological fluids
- Cross reactivity:Prostaglandin E1 ELISA Kit exhibits high specificity and excellent specificity for the detection of universal Prostaglandin E1. No significant cross-reactivity or interference between Prostaglandin E1 and analogues was observed.
- Detection method:Colorimetric
- Time to Results:2 hours
- Shelf life:Store for 6 months at 4 °C
- Detection range:31,25 - 2000 pg/ml
- Storage temperature:4 °C
- Sample volume:50 μl
- Sensitivity:18,75 pg/ml
- Regulatory status:RUO
- Tests per kit:96 tests
Prostaglandin E1 ELISA kit is a competitive Enzyme-Linked Immunosorbent Assay (cELISA) designed for the in vitro quantitative determination of Prostaglandin E1 in serum, plasma, tissue homogenates, and other biological fluids.
- Ready-To-Use ELISA kit
- Assay Precision: Intra-Assay: CV <8%, Inter-Assay: CV <10%
Prostaglandin E1 ELISA kit employs the competitive enzyme immunoassay technique for the quantitative measurement of Prostaglandin E1 in serum, plasma, tissue homogenates, and other biological fluids. The 96-well microtiter plate has been pre-coated with Prostaglandin E1 antigen. During the incubation, Prostaglandin E1 present in the samples or standards competes with the fixed amount of immobilised Prostaglandin E1 for binding sites on the Biotinylated Anti-Prostaglandin E1 Antibody. The more Prostaglandin E1 present in a sample or standard, the less Biotinylated Anti-Prostaglandin E1 Antibody that binds to the plate. Following incubation, unbound Biotinylated Anti-Prostaglandin E1 Antibody is removed by washing, and an HRP-Streptavidin conjugate is added to the wells and the microtiter plate is incubated. Following incubation and washing, TMB substrate solution is then used to visualise the HRP enzymatic reaction by catalysis to produce a blue-coloured product that changes to yellow after addition of acidic stop solution. The density of yellow is inversely proportional to the amount of Prostaglandin E1 present in each sample or standard. The concentration of Prostaglandin E1 can then be calculated by reading the O.D. absorbance at 450 nm in a microplate reader and referring to the standard curve.