- Assay duration:Multiple steps
- Assay Type:Sandwich
- Format:Pre-coated
- Primary antibody reactivity:Human
- Target protein:eIF5A
- Description:Human eIF5A ELISA kit
- Sample type:Serum, plasma, tissue homogenates, and other biological fluids
- Cross reactivity:Human eIF5A ELISA Kit exhibits high specificity and excellent specificity for the detection of human eIF5A. No significant cross-reactivity or interference between eIF5A and analogues was observed.
- Detection method:Colorimetric
- Time to Results:4 hours
- Shelf life:Store for 6 months at 4 °C
- Detection range:0,156 - 10 ng/ml
- Storage temperature:4 °C
- Sample volume:100 μl
- Sensitivity:0,094 ng/ml
- Regulatory status:RUO
- Tests per kit:96 tests
Human eIF5A ELISA kit is a sandwich Enzyme-Linked Immunosorbent Assay (sELISA) designed for the in vitro quantitative determination of human eIF5A in serum, plasma, tissue homogenates, and other biological fluids.
- Ready-To-Use ELISA kit
- Assay Precision: Intra-Assay: CV <8%, Inter-Assay: CV <10%
Human eIF5A ELISA kit employs the sandwich enzyme immunoassay technique for the quantitative measurement of human eIF5A in serum, plasma, tissue homogenates, and other biological fluids. An antibody specific for eIF5A has been pre-coated onto a 96-well microtiter plate. The standards and test samples are added into the wells and the eIF5A present in each sample is bound to the wells by the immobilised antibody. Following incubation, the wells are washed and then incubated with Biotinylated Anti-eIF5A Antibody, which binds the captured eIF5A present in each well. Following incubation, unbound biotinylated detection antibody is removed by washing, and an HRP-Streptavidin conjugate is added to the wells and the microtiter plate is incubated. Following incubation and washing, TMB substrate solution is then used to visualise the HRP enzymatic reaction by catalysis to produce a blue-coloured product that changes to yellow after addition of acidic stop solution. The density of yellow is proportional to the amount of eIF5A captured in each well. The concentration of eIF5A can then be calculated by reading the O.D. absorbance at 450 nm in a microplate reader and referring to the standard curve.