Phusion® High-Fidelity PCR Master Mix with HF Buffer
Phusion® High-Fidelity PCR Master Mix with HF Buffer is a convenient 2X mix containing Phusion® DNA Polymerase, nucleotides and optimised HF buffer including MgCl₂. Only template and primers need to be added by the user.
: Each kit contains Phusion® DNA Polymerase, 2X Phusion® HF Buffer and 400 µM of each dNTP. The master mix supplies 1,5 mM MgCl₂ and 200 µM dNTP in final reaction concentration. A separate tube of DMSO is provided with the product.