Interleukin-3 (IL-3) is a glycoprotein that regulates differentiation and enhances proliferation of a variety of hematopoietic precursor cells, mast cells, basophils, and NK cells.

IL-3 Culture Supplement, mouse, is used to culture a variety of IL-3 responsive cells, e.g., mast cells, basophils, natural killer cells, and several hematopoietic precursor cells.

Mouse supplement is tested for ability to promote proliferation of an IL-3 dependent mouse cell line (32-DCL) in serum-containing medium. Mouse recombinant supplement is shown to stimulate proliferation of IL-3 dependent murine cells (FDC-P1). All products are filtered (0.2 µm membrane) and tested and found negative for the presence of bacteria, fungi, and mycoplasma.