Methyl blue, Sigma-Aldrich®
Supplier: SIGMA ALDRICH MICROSCOPY
About this item
Methyl blue (C.l. 42780) is a triaminotriphenylmethane dye with each amino group carrying a monosulfonated phenyl substituent. It is also known as Water blue, Acid blue 93, Cotton blue, Poirriers blue, and Aniline blue water soluble.
- Methyl blue has been used to stain leaf disks for callose visualisation within the cells
- It has been used to incubate cells for 4',6-diamidino-2-phenylindole (DAPI)/septa staining
- Used as a marker for β-(1-3)-d-glucans or acidic phloroglucinol for the detection of callose and lignin
- Used in Mann's stain as it involves a methyl blue-eosin mixture
Synonyms: Aniline blue sodium salt, [[4-[bis[4-[(sulphophenyl)amino]phenyl]methylene]-2,5-cyclohexadien-1-ylidene]amino]-benzenesulphonic acid sodium salt (1:2), Poirriers blue, Cotton blue, Water blue, C.I. 42780, Aniline blue, water soluble, Disodium [[4-[bis[4-[(sulphonatophenyl)amino]phenyl]methylene]cyclohexa-2,5-dien-1-ylidene]amino]benzenesulphonate, Acid Blue 93
- CAS Number:28983-56-4
- Formula:C₃₇H₂₇N₃Na₂O₉S₃
- MDL Number:MFCD00058509
- Molecular Weight:799.81 g/mol
- Merck Index:13,06055
- :M-Clarity™ quality level = MQ200
- Flash Pt:>300 °C (Pensky-Martens) (closed cup)
- Storage Temperature:Ambient
- EINECS:249-352-9
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Product Details & Documents
Methyl blue (C.l. 42780) is a triaminotriphenylmethane dye with each amino group carrying a monosulfonated phenyl substituent. It is also known as Water blue, Acid blue 93, Cotton blue, Poirriers blue, and Aniline blue water soluble.
- Methyl blue has been used to stain leaf disks for callose visualisation within the cells
- It has been used to incubate cells for 4',6-diamidino-2-phenylindole (DAPI)/septa staining
- Used as a marker for β-(1-3)-d-glucans or acidic phloroglucinol for the detection of callose and lignin
- Used in Mann's stain as it involves a methyl blue-eosin mixture
Synonyms: Aniline blue sodium salt, [[4-[bis[4-[(sulphophenyl)amino]phenyl]methylene]-2,5-cyclohexadien-1-ylidene]amino]-benzenesulphonic acid sodium salt (1:2), Poirriers blue, Cotton blue, Water blue, C.I. 42780, Aniline blue, water soluble, Disodium [[4-[bis[4-[(sulphonatophenyl)amino]phenyl]methylene]cyclohexa-2,5-dien-1-ylidene]amino]benzenesulphonate, Acid Blue 93
- CAS Number:28983-56-4
- Formula:C₃₇H₂₇N₃Na₂O₉S₃
- MDL Number:MFCD00058509
- Molecular Weight:799.81 g/mol
- Merck Index:13,06055
- :M-Clarity™ quality level = MQ200
- Flash Pt:>300 °C (Pensky-Martens) (closed cup)
- Storage Temperature:Ambient
- EINECS:249-352-9
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Specification Test Results
- Appearance (colour):Purple to very dark purple
- Appearance (form):Powder or crystals
- Thin layer chromatogr.:Corresponds to standard
- Solubility (colour):0.01 g in 10 ml water
- Solubility (method):0.01 g in 10 ml water
- pH-transition:9.4
- Colour:Blue
- Wavelength (1) (UV):595.0 - 605.0 nm
- Extinction (1%/1 cm) (1) (UV):≥400
- Wavelength (2) (UV):310.0 - 320.0 nm
- Extinction (1%/1 cm) (2) (UV):≥200
- Solvent (UV):HCl 0.005 m