Animal component-free DNase I for digestion of DNA.

• Obtain cleaner cultures with an animal component-free solution
• Effectively eliminate DNA contaminants from dissociation medium

Use Deoxyribonuclease I (DNase I), Animal Component-Free (ACF) for routine tissue dissociation, to minimise cell clumping, and eliminate DNA contaminants from dissociation medium. Obtained from cultures free of animal-derived materials and purified by chromatography, this endonuclease consists of a single glycosylated polypeptide chain with two disulfide bonds. It preferentially cleaves phosphodiester linkages adjacent to pyrimidine nucleotides in both single- and double-stranded DNA, yielding polynucleotides with 5'-phosphate and 3'-hydroxyl groups (Bernardi et al.). DNase I has been used for DNA digestion in human cells and tissues such as microglia (Klegeris and McGeer), cartilage (Dunham and Koch), colon (Fukushima and Fiocchi), epithelium (Fukushima and Fiocchi), liver (Vatakis et al.), lung (Fujino et al.), neural cells (Fuja et al.), and stem cells (Kusuma et al.).