Human HPV Type 16 IgM Antibody ELISA kit is a 90 minutes Indirect Enzyme-Linked Immunosorbent Assay designed for the in vitro qualitative determination of human HPV Type 16 IgM Antibody in serum, plasma, and other biological fluids.

• Higher throughput: Get results in just 90 minutess, with a single wash step
• Sample Type: Serum, plasma or other biological fluids
• Assay Precision: Intra-Assay: CV <8%, Inter-Assay: CV <10%

Human HPV Type 16 IgM antibody ELISA kit employs the indirect enzyme-linked immunoassay technique for the qualitative detection of human HPV Type 16 IgM antibody in serum, plasma, and other biological fluids. The antigen recognised by the HPV Type 16 IgM antibody has been pre-coated onto a 96-well microtiter plate. Positive controls, negative controls, and samples are added into the wells and incubated. antibodies in the sample bind to the pre-coated antigen. Unbound antibody is removed in a wash step. An HRP-conjugated detection antibody is then added and incubated. Unbound HRP-conjugated detection antibody is removed in another wash step. TMB substrate is then added, and colour develops. The reaction is terminated by the addition of acidic stop solution and the intensity of the yellow colour is measured at O.D. 450 nm in a microplate reader. The O.D. 450 nm of samples is then compared to that of the positive and negative controls to determine the presence or absence of human HPV Type 16 IgM antibody.