Mouse ACE2 ELISA Kit

STMC100-0688

: STEMCELL Technologies

Mouse ACE2 ELISA Kit

STMC100-0688

: STEMCELL Technologies

Pk:
1 KIT
Target protein:
Mouse ACE2

For detection and measurement of mouse ACE2.

The Mouse ACE2 ELISA Kit is designed for the quantitative detection and measurement of mouse angiotensin converting enzyme 2 (ACE2) in biological fluids such as serum, plasma, cell culture supernatant, and tissue homogenate. ACE2 is a type I integral membrane protein. It is highly expressed in capillary-rich organs such as the lungs, heart, blood vessels, kidneys, liver, and gastrointestinal tract.

The ACE2 receptor is the target of the SARS-CoV-2 Spike (S) glycoprotein. This interaction allows for viral entry into the host cell, making ACE2 a potential target for antiviral therapeutics. The assay is based on the sandwich ELISA method, in which samples are added to ELISA strip plates pre-coated with capture antibodies specific for ACE2.

The captured ACE2 is detected by addition of a biotinylated detection antibody, followed by streptavidin-horseradish peroxidase, which binds the biotinylated antibody. Addition of the chromogenic enzyme substrate 3,3’, 5,5’ tetramethylbenzidine (TMB) results in a coloured product with an intensity directly proportional to the concentration of ACE2 in the sample.

The concentration of ACE2 is determined by comparison to a serial dilution of the standard analysed in parallel.

: Product restrictions: (i) Customer shall not make products or any portion of them in any way, shape or form, including as a component of another product available for the purpose of further resale or alter or remove the Product label and the Supplier’s mark of origin without express written permission of Supplier and (ii) Customer is familiar with Supplier’s restrictions on the use of the products and shall use the products in compliance with such restrictions and the applicable Supplier Product Information sheet (collectively, the 'Product Restrictions').