Anti-NSUN2 Rabbit Polyclonal Antibody
Catalog # PRSI55-438
Supplier: ProSci Inc.
Specifications
- Antibody type:Primary
- Antigen name:tRNA (cytosine(34)-C(5))-methyltransferase
- Antigen symbol:NSUN2
- Clonality:Polyclonal
- Conjugation:Unconjugated
- Host:Rabbit
- Western blot:Yes
- Epitope:Amino Acid Range = 423-451
- Form:Liquid
- Gene ID:Q08J23
- Antigen synonyms:NSUN2
- Modification:None
- Storage buffer:Supplied in PBS with 0,09% (W/V) sodium azide
- Molecular weight:86 kDa
- Storage temperature:Store at 4 °C for three months and –20 °C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
- Concentration:0,5 mg/ml
- Shipping temperature:4 °C
- Immunogen:This NSUN2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 423-451 amino acids from the Central region of human NSUN2.
- Purification:This antibody is purified through a protein A column, followed by peptide affinity purification.
- Size:400 µl
- Pk:400 µl
Specifications
About this item
Maturation of cytoplasmic tRNAs includes splicing of introns, which are located 1 nucleotide 3-prime from the anticodon in all intron-containing tRNA genes. In tRNA-leu(CAA), the first position of the anticodon, C34, is converted to 5-methylcytosine, a modification necessary to stabilize the anticodon-codon pairing and correctly translate the mRNA. NSUN2 encodes a methyltransferase that catalyzes the intron-dependent formation of 5-methylcytosine at C34 of tRNA-leu(CAA) (Brzezicha et al., 2006 [PubMed 17071714]).
For WB starting dilution is: 1:1000.
Type: Primary
Antigen: NSUN2
Clonality: Polyclonal
Clone:
Conjugation: Unconjugated
Epitope:
Host: Rabbit
Isotype: Rabbit Ig
Reactivity: Human, Mouse