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Anti-NSUN2 Rabbit Polyclonal Antibody
Anti-NSUN2 Rabbit Polyclonal Antibody
Catalog # PRSI55-438
Supplier:  ProSci Inc.
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Anti-NSUN2 Rabbit Polyclonal Antibody
Catalog # PRSI55-438
Supplier:  ProSci Inc.

Specifications

  • Antibody type:
    Primary
  • Antigen name:
    tRNA (cytosine(34)-C(5))-methyltransferase
  • Antigen symbol:
    NSUN2
  • Clonality:
    Polyclonal
  • Conjugation:
    Unconjugated
  • Host:
    Rabbit
  • Western blot:
    Yes
  • Epitope:
    Amino Acid Range = 423-451
  • Form:
    Liquid
  • Gene ID:
    Q08J23
  • Antigen synonyms:
    NSUN2
  • Modification:
    None
  • Storage buffer:
    Supplied in PBS with 0,09% (W/V) sodium azide
  • Molecular weight:
    86 kDa
  • Storage temperature:
    Store at 4 °C for three months and –20 °C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
  • Concentration:
    0,5 mg/ml
  • Shipping temperature:
    4 °C
  • Immunogen:
    This NSUN2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 423-451 amino acids from the Central region of human NSUN2.
  • Purification:
    This antibody is purified through a protein A column, followed by peptide affinity purification.
  • Size:
    400 µl
  • Pk:
    400 µl

Specifications

About this item

Maturation of cytoplasmic tRNAs includes splicing of introns, which are located 1 nucleotide 3-prime from the anticodon in all intron-containing tRNA genes. In tRNA-leu(CAA), the first position of the anticodon, C34, is converted to 5-methylcytosine, a modification necessary to stabilize the anticodon-codon pairing and correctly translate the mRNA. NSUN2 encodes a methyltransferase that catalyzes the intron-dependent formation of 5-methylcytosine at C34 of tRNA-leu(CAA) (Brzezicha et al., 2006 [PubMed 17071714]).

For WB starting dilution is: 1:1000.

Type: Primary
Antigen: NSUN2
Clonality: Polyclonal
Clone:
Conjugation: Unconjugated
Epitope:
Host: Rabbit
Isotype: Rabbit Ig
Reactivity: Human, Mouse