Specifications
- Antibody type:Primary
- Antigen name:integrin, beta 3 (platelet glycoprotein IIIa, antigen CD61)
- Antigen symbol:ITGB3
- Clonality:Monoclonal
- Clone:Y2/51
- Conjugation:Unconjugated
- Flow cytometry:Yes
- Host:Mouse
- ImmunoChemistry:Yes
- ImmunoFluorescence:Yes
- Isotype:IgG1 kappa
- Western blot:Yes
- Format:Purified Antibody
- Form:Liquid
- Gene ID:P05106
- Antigen synonyms:ITGB3
- Storage buffer:PBS with 0,1 mg/ml BSA and 0,05% sodium azide
- Storage temperature:Aliquot and Store at 2…8 °C. Avoid freez-thaw cycles.
- Concentration:0,2 mg/ml
- Shipping temperature:4 °C
- Immunogen:Phytohemagglutinin (PHA)-stimulated peripheral blood cells were used as the immunogen for the CD61 antibody.
- Purification:Protein G affinity
- Size:100 µg
- Pk:100 µG
Specifications
About this item
Reacts with human CD61, also called Integrin beta 3 (GPIIIa, vitronectin receptor beta chain). It associates with the alphaV-chain (CD51) to form vitronectin receptor, or with the alphaIIb-chain (CD41) to form the GpIIb/GpIIIa complex (CD41/CD61). The CD41/CD61 complex appears early in megakaryocyte maturation. The activated CD41/CD61 complex is a receptor for von Willebrand factor, soluble fibrinogen, fibronectin, vitronectin and thrombospondin. It plays a central role in platelet activation and aggregation. The CD51/CD61 is implicated in tumor metastasis and adenoviral infection. The antibody detects platelets in smears of blood and bone marrow, as well as megakaryocytes in frozen sections and cell smears. The antibody is useful for classification of megakaryoblastic leukemia.
Flow Cytometry: 0,5-1 µg/million cells in 0,1 ml Immunofluorescence: 0,5 - 1 µg/ml IHC (FFPE): 1 - 2 µg/ml for 30 min at RT Titration of the CD61 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Type: Primary
Antigen: ITGB3
Clonality: Monoclonal
Clone: Y2/51
Conjugation: Unconjugated
Epitope:
Host: Mouse
Isotype: IgG1, kappa
Reactivity: Human