Specifications
- Antibody type:Primary
- Antigen name:CD55 molecule, decay accelerating factor for complement (Cromer blood group)
- Antigen symbol:CD55
- Clonality:Monoclonal
- Clone:F4-29D9
- Conjugation:Unconjugated
- Flow cytometry:Yes
- Host:Mouse
- ImmunoFluorescence:Yes
- Isotype:IgG1 kappa
- Reactivity:Human
- Western blot:Yes
- Format:Purified Antibody
- Form:Liquid
- Gene ID:P08174
- Antigen synonyms:CD55
- Storage buffer:PBS with 0,1 mg/ml BSA and 0,05% sodium azide
- Storage temperature:Aliquot and Store at 2…8 °C. Avoid freez-thaw cycles.
- Concentration:0,2 mg/ml
- Shipping temperature:4 °C
- Immunogen:Human umbilical vein endothelial cells (HUVEC) were used as the immunogen for the CD55 antibody.
- Purification:Protein G affinity chromatography
- Size:100 µg
- Pk:100 µG
Specifications
About this item
Recognises a single chain glycoprotein of 70 kDa, identified as CD55 (also known as decay accelerating factor, DAF). This mAb was clustered in Kobe at the Sixth International Workshop on Human Leukocyte Differentiation Antigens as F429D-9 (N-L120). CD55/DAF is widely expressed on cells throughout the body including leukocytes, erythrocytes, epithelium, endothelium, and fibroblasts. It is a Glycosyl phosphatidylinositol anchored (GPI-anchored) member of the membrane bound complement regulatory proteins that inhibit autologous complement cascade activation. It prevents the amplification steps of the complement cascade by interfering with the assembly of the C3-convertases, C4b2a and C3bBb, and the C5-convertase, C4b2a3b and C3bBb3b. CD55 also serves as receptor for CD97 and for echovirus and Coxsackie B virus. Anti-CD55 can be used as marker for paroxysmal nocturnal hemoglobinuria (PNH).
Flow Cytometry: 0,5-1 µg/million cells in 0,1 ml Immunofluorescence: 1 - 2 µg/ml Optimal dilution of the CD55 antibody should be determined by the researcher.
Type: Primary
Antigen: CD55
Clonality: Monoclonal
Clone: F4-29D9
Conjugation: Unconjugated
Epitope:
Host: Mouse
Isotype: IgG1, kappa
Reactivity: Human