Human shRNA plasmid kit, ANKRD55 is designed against multiple splice variants at this gene locus.

• Vector: pGFP-C-shLenti (TR30023)
• Antibiotic selection: Chloramphenicol (34 µg/ml))

OriGene guarantees that the sequences in the shRNA expression cassettes are verified to correspond to the target gene with 100% identity. One of the four constructs at minimum are guaranteed to produce 70% or more gene expression knock-down provided a minimum transfection efficiency of 80% is achieved. Western Blot data is recommended over qPCR to evaluate the silencing effect of the shRNA constructs 72 hrs post transfection. To properly assess knockdown, the gene expression level from the included scramble control vector must be used in comparison with the target-specific shRNA transfected samples.