DENARASE High Salt is an engineered version of the wild-type Serratia marcescens endonuclease, which is commonly used for DNA removal applications in bioprocessing. Compared to the wild-type DENARASE, a few amino acids were substituted to provide the enzyme with an improved salt tolerance without losing its specificity for nucleic acids.

• Designed for improved salt and pH tolerance: For highest process flexibility and efficiency
• Tailored to fit your process: Seamless integration into existing processes
• Compatible with DENARASE ELISA Kit: The one-for-all Serratia marcescens ELISA Kit

Like the wild-type S. marcescens endonuclease, DENARASE High Salt is capable of the non-specific cleavage of phosphodiester bonds between the nucleotide subunits of nucleic acids. The enzyme can hydrolyze all forms of DNA and RNA, such as single-stranded, double-stranded, linear, circular, or supercoiled.
The protein consists of two monomers with a calculated molecular weight of 27 kDa each. The calculated isoelectric point (pI) of DENARASE High Salt is at pH 7.83. Magnesium (Mg2+) is an essential co-factor and prerequisite for the nucleic acid clearance activity of the salt-tolerant endonuclease.

Similar to the wild-type DENARASE, the manufacturing process of DENARASE High Salt is based on recombinant expression in a Bacillus sp. production host. The gram-positive production strain is free of endotoxins. DENARASE High Salt is manufactured and supplied by c-LEcta GmbH.

DENARASE High Salt enables optimal and cost-effective removal of nucleic acids in biomanufacturing processes that benefit from higher salt concentrations. The enzyme is especially suitable for the use at bioprocessing-relevant 200-500 mM salt and pH 7.4-pH 8. It is designed to improve the efficiency of various applications:
Viral Vaccines
Viral Vectors for Cell and Gene Therapies
Viscosity Reduction in Lysates
Sample Preparation in Electrophoresis and Chromatography

Enzyme origin: Engineered from Serratia marcescens
Production host: Bacillus sp.
Temperature optimum: 37 °C
Magnesium optimum: 5 - 25 mM
Isoelectrical point: pH 7.83
Salt: 200 - 500 mM