Specifications
- Media Format:Powder
- Pk:1
- Description:RPMI 1640 medium without L-glutamine, L-serine, methionine, HEPES (powder)
- Size:10 L
- HEPES:Yes
- Phenol red:Yes
- L-Glutamine:No
- Glucose level:High
- Media Base:RPMI 1640 - Roswell Park Memorial Institute Medium
- Storage temperature:Store powdered media at RT. Stable for 12 months after receipt. Opened bottles should be capped tightly and kept in a dark, low humidity environment. Prepared media should be kept at 4 °C and used within a short period of time.
- Shipping temperature:Powder, RT
Specifications
About this item
RPMI-1640 was developed by Moore, et. al. at Roswell Park Memorial Institute, hence the acronym RPMI. The formulation is based on the RPMI-1630 series of media utilizing a bicarbonate buffering system and alterations in the amounts of amino acids and vitamins. RPMI-1640 medium has been used for the culture of human normal and neoplastic leukocytes. RPMI-1640, when properly supplemented, has demonstrated wide applicability for supporting growth of many types of cultured cells, including fresh human lymphocytes in the 72 hour phytohemaglutinin (PHA) stimulation assay.
- Appearance: Pale pink, homogenous, free flowing powder
- Solubility: Magenta to red, clear, complete
- Endotoxin: ≤1 EU/ml
Directions per Liter: Dissolve 10,09 g in 900 ml of ddH₂O, stirring until completely solubilised. Do NOT heat. Add 2 g sodium bicarbonate with stirring. Adjust pH of the medium to 0,1 to 0,3 pH units below the desired pH. Add additional water to bring the solution to final volume. Filter-sterilise using a 0,22 µm membrane. Aliquot into sterile containers.