This cADP-ribose assay can be performed in a convenient 96-well or 384-well microtiter plate.

• In-house QC testing
• Sensitive
• Suggested protocol available

cADP-ribose (cADPR) is a novel Ca2+ messenger derived from NAD+. ADP-riboxyl cyclase (ADPRC) catalyses the synthesis of cADPR from NAD+, but the reaction can be reversed in the presence of high concentration of nicotinamide, producing NAD+ from cADPR stoichiometrically.

The resultant NAD+ can be detected using our newly developed NAD sensor Quest Fluor™ NAD reagent. This makes monitoring cADPR in tissues and cell cultures possible in the low nM range.

The NAD+ detection using Quest Fluor™ NAD reagent is specific to NAD+ and has no reaction to NADH. The fluorescent signal can be readily detected.