Proteinase K Solution

Proteinase K from the fungus engyodontium album is a nonspecific serine protease that is useful for general digestion of proteins. Proteinase K remains active.

• Over a wide pH range optimal activity between 6,5 and 9,5
• Under denaturing conditions e.g., in the presence of SDS or urea
• In the presence of metal chelating agents e.g., EDTA
• At comparatively high temperatures optimum digestion temperature is 65 °C

Removal of endogenous nucleases during the preparation of DNA and RNA, preparation of tissue sections for in situ hybridisation. Endodeoxyribonuclease and exodeoxyribonuclease assays; liquid form tested for absence of RNase activity.

Caution: For research use only. Not for use in diagnostic procedures.