Affinity chromatography columns, HisTrap™ HP
About this item
HisTrap HP prepacked columns provide a fast, convenient and reproducible format for preparative purification of histidine-tagged (His-tag) recombinant proteins.
- High resolution: Due to small bead size (average bead size is 34 µm)
- High binding capacity: At least 40 mg His tag protein per mL resin, for high yields
- Negligible nickel leakage: Helps retain activity and minimize protein precipitation
- Broad reagent compatibility: Suitable for use with a wide range of reducing agents, detergents, denaturants, and other additives
- Convenient HiTrap format: 1 ml and 5 ml columns compatible with syringe, pump, or chromatography systems such as ÄKTA or other FPLC systems
His tag protein purification by IMAC is both popular and highly efficient. HisTrap HP columns are packed with Ni Sepharose High Performance (HP) affinity resin. This resin consists of highly cross-linked agarose beads to which a chelating group has been coupled. The chelating group is precharged with nickel, which selectively retains proteins with exposed histidine groups.
HisTrap HP IMAC resin has a binding capacity that exceeds 40 mg/ml, resulting in high protein recovery. The small bead size (34 µm) contributes to a purer purification compared to nickel resins of larger bead size.
4 Options Available Below
Product Details & Documents
HisTrap HP prepacked columns provide a fast, convenient and reproducible format for preparative purification of histidine-tagged (His-tag) recombinant proteins.
- High resolution: Due to small bead size (average bead size is 34 µm)
- High binding capacity: At least 40 mg His tag protein per mL resin, for high yields
- Negligible nickel leakage: Helps retain activity and minimize protein precipitation
- Broad reagent compatibility: Suitable for use with a wide range of reducing agents, detergents, denaturants, and other additives
- Convenient HiTrap format: 1 ml and 5 ml columns compatible with syringe, pump, or chromatography systems such as ÄKTA or other FPLC systems
His tag protein purification by IMAC is both popular and highly efficient. HisTrap HP columns are packed with Ni Sepharose High Performance (HP) affinity resin. This resin consists of highly cross-linked agarose beads to which a chelating group has been coupled. The chelating group is precharged with nickel, which selectively retains proteins with exposed histidine groups.
HisTrap HP IMAC resin has a binding capacity that exceeds 40 mg/ml, resulting in high protein recovery. The small bead size (34 µm) contributes to a purer purification compared to nickel resins of larger bead size.
Documents
17524701_-HAND BOOK 1 17524701_HAND BOOK 17524701_HAND BOOK 2 17524701_HAND BOOK 3 17524701_INSTRUCTIONS
Manual
17524701_DATA FILE 17524701_DATA FILE 1 17524705_DATA FILE 17524705_DATA FILE 1 17524801_DATA FILE
Datasheet
Cytiva CyDye Licensing Statements Cytiva General Licensing Statements
Additional Product Details
Specifications for Product Family
Specifications
- Catalog No:
- 29-0510-21
- 17-5247-01
- 17-5247-05
- 17-5248-01
- 17-5248-02
- Binding capacity:
- At least 40 mg histidine-tagged protein/ml medium
- At least 40 mg histidine-tagged protein/ml medium
- At least 40 mg histidine-tagged protein/ml medium
- At least 40 mg histidine-tagged protein/ml medium
- At least 40 mg histidine-tagged protein/ml medium
- Max. pressure:
- 5 bar
- 5 bar
- 5 bar
- 5 bar
- 5 bar
- pH range:
- 3 - 12
- 3 - 12
- 3 - 12
- 3 - 12
- 3 - 12
- Storage:
- 4 to 30 °C, 20% Ethanol
- 4 to 30 °C, 20% Ethanol
- 4 to 30 °C, 20% Ethanol
- 4 to 30 °C, 20% Ethanol
- 4 to 30 °C, 20% Ethanol
Specifications
Product Family Options
Product Information
- Column hardwareParticle SizeBed dimensionsCapacityFlow rateAvailabilityPrice
- 29-0510-21HisTrap™ HPSpecifications:
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Specifications
Cat. No.29-0510-21Binding capacityAt least 40 mg histidine-tagged protein/ml mediumMax. pressure5 barpH range3 - 12Storage4 to 30 °C, 20% Ethanol - 17-5247-01HisTrap™ HPSpecifications:
More
Specifications
Cat. No.17-5247-01Binding capacityAt least 40 mg histidine-tagged protein/ml mediumMax. pressure5 barpH range3 - 12Storage4 to 30 °C, 20% Ethanol - 17-5247-05HisTrap™ HPHisTrap™ HPPP34 µm7×25 mm1 ml4 ml/minDiscontinued-Specifications:
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Specifications
Cat. No.17-5247-05Binding capacityAt least 40 mg histidine-tagged protein/ml mediumMax. pressure5 barpH range3 - 12Storage4 to 30 °C, 20% Ethanol - 17-5248-01HisTrap™ HPSpecifications:
More
Specifications
Cat. No.17-5248-01Binding capacityAt least 40 mg histidine-tagged protein/ml mediumMax. pressure5 barpH range3 - 12Storage4 to 30 °C, 20% Ethanol - 17-5248-02HisTrap™ HPSpecifications:
More
Specifications
Cat. No.17-5248-02Binding capacityAt least 40 mg histidine-tagged protein/ml mediumMax. pressure5 barpH range3 - 12Storage4 to 30 °C, 20% Ethanol