- Antibody type:Primary
- Antigen name:Lin-28
- Clonality:Polyclonal
- Conjugation:Alexa Fluor® 680
- Host:Rabbit
- ImmunoChemistry:Yes
- ImmunoFluorescence:Yes
- Isotype:IgG
- Reactivity:Human,Rat
- Western blot:Yes
- Epitope:65-160/250
- Form:Liquid
- Gene ID:389421, 79727
- Antigen synonyms:LIN28B|LN28B_HUMAN|Lin-28B|Lin28B|Lin 28.2|CSDD 2|Protein lin-28 homolog A, Lin-28A, Zinc finger CCHC domain-containing protein 1|CSDD2|FLJ16517|Protein lin-28 homolog B|Lin 28 homolog B C. elegans
- Modification:Unmodified
- Storage buffer:Aqueous buffered solution containing 0,01 M TBS (pH 7,4) with 1% BSA, 0,03% Proclin300 and 50% Glycerol
- Molecular weight:27 kDa
- Storage temperature:Store at ‒20 °C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
- Concentration:1 µg/µl
- Shipping temperature:4 °C
- Immunogen:KLH conjugated synthetic peptide derived from human Lin-28B
- Tested applications:ICC
- Purification:Purified by Protein A
- Pack type:Vial
- Pk:100 µl
Acts as a suppressor of microRNA (miRNA) biogenesis by specifically binding the precursor let-7 (pre-let-7), a miRNA precursor. Acts by binding pre-let-7 and recruiting ZCCHC11/TUT4 uridylyltransferase, leading to the terminal uridylation of pre-let-7. Uridylated pre-let-7 miRNAs fail to be processed by Dicer and undergo degradation. Specifically recognises the 5'-GGAG-3' motif in the terminal loop of pre-let-7. Also recognises and binds non pre-let-7 pre-miRNAs that contain the 5'-GGAG-3' motif in the terminal loop, leading to their terminal uridylation and subsequent degradation. Mediates MYC-mediated let-7 repression. Isoform 1, when overexpressed, stimulates growth of the breast adenocarcinoma cell line MCF-7. Isoform 2 has no effect on cell growth.
Type: Primary
Antigen: Lin-28
Clonality: Polyclonal
Clone:
Conjugation: ALEXA FLUOR® 680
Public Immunogen Range: 65-160/250
Host: Rabbit
Isotype: IgG
Reactivity: Human; Rat