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Propidium Iodide

Propidium Iodide
 
Propidium Iodide
Propidium Iodide

 

 

Propidium Iodide (PI) is a membrane-impermeant DNA binding dye that cannot penetrate viable cells. PI rapidly enters cells with compromised membranes and intercalates between base pairs allowing exclusion of non-viable cells from analysis of flow cytometry data. PI has a broad emission spectrum from 535-617 nm and can be detected in either FL2 or FL3 detectors. When used with Annexin V FITC, it is recommended to analyze PI in FL2.

  • Formulation: 10 mM NaH₂PO₄, 150 mM NaCl, 0,09% NaN₃, pH 7,2
 :  3,8-Diamino-5-[3-(diethylmethylammonio)propyl]-6-phenylphenanthridinium diiodide, PI, 3,8-Diamino-5-(3-(diethyl(methyl)ammonio)propyl)-6-phenylphenanthridin-5-ium iodide, 3,8-Diamino-5-(3-diethylaminopropyl)-6-phenylphenanthridinium iodide methiodide
  •  :25535-16-4
  •  :C₂₇H₃₄I₂N₄
  •  :MFCD00011921
  •  :668.4 g/mol
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