Quality control of BAC digests and large DNA fragments using pulsed-field capillary electrophoresis.

• Reliable sizing - Accurate and precise sizing of large DNA fragments from 75 bp to 48 kb or 75 bp to 165 kb
• High resolution separations - Optimised pulsed-field method prevents peak broadening, allowing separation of similarly sized large DNA fragments
• Flag analysis - ProSize data analysis software allows for automated flagging of DNA fragments decreasing analysis times
• Low sample volume - Only 2 µl required, conserving sample for downstream analysis
• Fast separation time - Quality control results in approximately 1,5 hours

The new 165 kb BAC kit for the Femto Pulse system is designed for the pulse-field CE separation and sizing of large bacterial artificial chromosome (BAC) fragment. The new kit provides unparalleled fragment resolution, sensitivity, and accuracy for sizing high molecular weight DNA fragments such as BAC libraries ranging from 75 to 165000 bp in size, with single-fragment detection sensitivity down to 1,6 pg/μl and up to 50 pg/μl.

The 55 kb BAC kit for the Agilent Femto pulse system is designed for quality control of BAC digests and large DNA fragments with accurate sizing up to 55 kb. A highly optimised pulsed-field method prevents peak broadening, ensuring high-resolution and accurate sizing of BAC digests and large DNA fragments in approximately 1,5 hours.