Fetal Bovine Serum often contains the antibiotic tetracycline (tet) and researchers using very sensitive tet-inducible gene expression systems encounter problems with unintended expression as a result.

• Sera is aseptically collected and processed in a cGMP facility
• Each batch of raw serum is screened for endotoxin, residual hemoglobin concentration, and microbiological quality before being used in production
• Batches of raw serum are pooled and continuously mixed prior to filtration to ensure consistent quality throughout the entire lot
• Final lots are tested for endotoxin, hemoglobin, pH, mycoplasma, and growth promotion. Biochemical assays and virus testing are also available upon request
• Tetracycline Negative FBS is sourced from US herds

Fetal Bovine Serum often contains the antibiotic tetracycline (tet) and researchers using very sensitive tet-inducible gene expression systems encounter problems with unintended expression as a result. Serum containing minimal levels of tetracycline is needed to ensure accurate gene expression. The tetracycline test is performed either with the ELISA method or bioassay methodology techniques. The limit of detection is approximately 5 µg/ml. Tetracycline negative FBS is used in applications where the presence of tetracycline would prove disruptive.