The fastest and latest generation of the market-leading QuikChange kits speeds up the protocol for performing single and multiple site-directed mutagenesis to less than 3 hours.
- For single site mutagenesis, one kit accommodates both short and long templates (4 to 14 kB)
- >80% mutagenesis efficiency for 1 site
- >55% mutagenesis efficiency for 3 mutations simultaneously
These QuikChange Lightning kits, like their predecessors, are based on a linear amplification method whereby mutation-containing primers are incorporated but not copied.
The protocols of other commercially available kits rely on PCR-based methods, which may result in unwanted second-site errors. The QuikChange Lightning Kit allows you to introduce point mutations, insertions, or deletions twice as fast compared to earlier kit versions, and the QuikChange Lightning Multi Kit delivers mutants up to three times faster than our original multi-site mutagenesis kit, both kits accomplish this without sacrificing the mutagenesis efficiency, reliability and accuracy.