Live-or-Dye NucFix™ Red is a red fluorescent dye to covalently label dead cells, allowing cells with permeable plasma membranes to be detected by flow cytometry or fluorescence microscopy.
- Nuclear-specific dead cell stain
- Highly dead-cell specific
- Covalent labeling with reactive dye
- Withstand fixation and permeabilization
- Selective dead-cell staining of mammalian cells, yeast, or gram-negative bacteria
Live-or-Dye NucFix™ Red is a reactive, cell membrane impermeant, red fluorescent dye that specifically stains the nuclei of dead cells. The dye is able to enter into dead cells that have compromised membrane integrity and covalently label the cell nucleus, allowing for clear differentiation of live and dead cells by either microscopy or flow cytometry. Unlike other commonly used nuclear stains such as Propidium Iodide (PI) or DRAQ7™, NucFix™ labeling is extremely stable, allowing the cells to be fixed and permeabilized without loss of fluorescence or dye transfer between cells. The Live-or-Dye NucFix™ Red staining protocol has been optimized to maximize live/dead discrimination with minimal live cell staining, in order to prevent interference with immunostaining. The dye has excitation/emission at 520/593 nm (with DNA).