Broad range RNA quantitation kits, AccuBlue®

The AccuBlue® broad range RNA quantitation kit boasts the widest linear range (5 to 1000 ng RNA) of available RNA quantitation kits, while providing exceptional accuracy, sensitivity, and high RNA selectivity.

Detection range spans Qubit® HS and BR
Highly selective for RNA over dsDNA
Far-red fluorescence emission
For fluorescence microplate reader or Qubit® RNA broad range program
Mammalian RNA standard to accurately quantify similar samples

High sensitivity and broad linear range:
The assay is linear between 5 and 1000 ng of RNA per 200 μl assay well, which spans both Qubit® high sensitivity and Qubit® broad range detection ranges. It is ideal for use in quantifying RNA for sensitive applications such as Next-Gen Sequencing (NGS) or reverse transcription PCR (RT-PCR).

RNA specificity:
Unlike absorbance-based measurements, RNA broad range dye is selective for RNA over double-stranded DNA, and can tolerate an equimolar amount of dsDNA in the sample without significant effect on RNA quantitation (purified RNA samples are still recommended).

Instrument compatibility:
The AccuBlue® broad range RNA quantitation assay is designed for use with fluorescence microplate readers equipped with excitation and emission filters for detecting far-red fluorescence. It is also optimised for use in the Qubit® fluorometer, using the pre-programmed RNA Broad Range program.

: Replacement vials of RNA standard can be purchased separately.

: Kit components: RNA broad range dye 200X, RNA broad range buffer, RNA dilution buffer (Standard 1 if using Qubit®), RNA broad range standard, 100 ng/μl (Standard 2 if using Qubit®).