Affinity chromatography columns, HiTrap™ MabSelect SuRe™ pcc

HiTrap MabSelect SuRe PCC chromatography columns are prepacked with MabSelect SuRe™ pcc protein A resin for screening and small-scale mAb purification in continuous bioprocessing using periodic counter-current chromatography (PCC).

• Convenient prepacked column for fast, reproducible, and easy small-scale purifications
• High binding capacity, ~ 60 mg human IgG/ml resin at 2,4 min residence time
• High productivity through capture of large mass of monoclonal antibodies in a short period of time
• Highly concentrated elution pools for operating flexibility and small unit operations
• Allows cost-effective cleaning with 0,1 to 0,5 M NaOH over hundreds of purification cycles
• High protein ligand stability reduces ligand leakage

HiTrap MabSelect SuRe PCC packed columns (1 and 5 ml) are suited for small scale antibody purifications, as well as optimization of methods and parameters, such as sample load and protein binding conditions. These protein A columns provide fast, reproducible, and easy separations in a convenient format. The columns are optimally utilized when they are connected to liquid chromatography systems such as the AKTA systems.

The alkali-stabilised, protein A-derived ligand gives the resin greater stability than conventional protein A-based resins under the alkaline conditions used in cleaning-in-place (CIP) protocols. Consequently, the possibility of cleaning with cost-effective reagents such as sodium hydroxide (NaOH) will improve process economy.

In addition, the use of NaOH for cleaning efficiently inactivates bacteria, mold, and yeast, with improved product quality as a result.