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p53 transcription factor assay kit
p53 transcription factor assay kit
Catalog # CAYM600020-96
Supplier:  Cayman Chemical
CAS Number:  
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p53 transcription factor assay kit
Catalog # CAYM600020-96
Supplier:  Cayman Chemical
CAS Number:  

Specifications

  • Pk:
    1 KIT
  • Description:
    p53 transcription factor assay kit, 96 strip wells
  • Environmentally Preferable:

Specifications

About this item

96-Well assay for measurement of p53.

  • A sensitive, non-radioactive method of detecting p53 from whole cell lysates
  • 96-well plate format replaces EMSAs
  • Capture the transcription factor using a specific dsDNA sequence bound to the plate
  • Detect the dsDNA-bound transcription factor with specific antibodies in an ELISA format
  • Nuclear extraction kit is available to aid in the isolation of nuclear and cytoplasmic fractions from cell lysates or tissue homogenates

The tumor suppressor protein p53 plays a crucial role in coordinating cellular responses to genotoxic stress and holds many important clinical implications in the treatment of cancer. Cayman’s p53 Transcription Factor Assay is a non-radioactive, sensitive method for detecting specific transcription factor DNA binding activity in nuclear extracts. A specific double-stranded DNA (dsDNA) sequence containing the p53 response element is immobilized onto the wells of a 96-well plate. p53 contained in a nuclear extract, binds specifically to the p53 response element and is detected by addition of a specific primary antibody directed against p53. A secondary antibody conjugated to HRP is added to provide a sensitive colorimetric readout at 450 nm.

Delivery: Kit contains buffer, reagent A, positive control, binding buffer, primary antibody, buffer concentrate, polysorbate 20, competitor dsDNA, HRP conjugate, plate and cover sheet, developing and stop solution.