Human HO-1/HMOX1 PicoKine™ ELISA kit
Catalog # BSBTEK0888
Supplier: Boster Bio
CAS Number:
Specifications
- Pk:1 KIT
- Primary antibody reactivity:Human
- Target protein:HO-1/HMOX1
- Description:Human HO-1/HMOX1 PicoKine™ ELISA kit
- Sample type:Cell culture supernates, cell lysates, serum and plasma (heparin, EDTA)
- Cross reactivity:No detectable cross-reactivity
- Storage temperature:4 °C (6 months)
- Sensitivity:<10 pg/ml
Specifications
About this item
Human HO-1/HMOX1 PicoKine™ ELISA Kit is a high sensitivity ELISA kit for quantitative detection of human HO-1/HMOX1.
- Assay range: 93,7 pg/ml to 6000 pg/ml
- Validated species: human
- Capture antibody: monoclonal antibody from mouse
- Detection antibody: polyclonal antibody from goat
- Tissue specificity: expressed at higher levels in renal cancer tissue than in normal tissue (at protein level)
- Subcellular localisation: microsome endoplasmic reticulum membrane, peripheral membrane protein, cytoplasmic side
Heme oxygenase cleaves the heme ring at the alpha methene bridge to form biliverdin. Biliverdin is subsequently converted to bilirubin by biliverdin reductase. Under physiological conditions, the activity of heme oxygenase is highest in the spleen, where senescent erythrocytes are sequestrated and destroyed. Exhibits cytoprotective effects since excess of free heme sensitises cells to undergo apoptosis.
Human HO-1/HMOX1 PicoKine™ ELISA kit have 96 wells with removable strips.
Delivery: Kit includes 96-well plate precoated with anti-human HMOX1 antibody, lyophilised recombinant human HMOX1 standard (10 ng/tubex2), biotinylated anti-human HMOX1 antibody, 130 µl (dilution 1:100), avidin-biotin-peroxidase complex (ABC), 130 µl (dilution 1:100), sample diluent buffer (30 ml), antibody diluent buffer (12 ml), ABC diluent buffer (12 ml), TMB color developing agent (10 ml), TMB stop solution (10 ml) and adhesive cover (4).