Specifications
- Pk:1 KIT
- Assay Type:Direct
- Primary antibody reactivity:Species independent
- Target protein:cAMP
- Description:Direct cAMP ELISA kit
- Seq:02
- Storage conditions:4 °C (long term), −20 °C (standard and conjugate)
- Detection method:Colorimetric
- Detection range:0,78 - 200 pmol/ml (non-acetylated); 0,078 - 20 pmol/ml (acetylated)
- Sensitivity:0,39 pmol/ml (non-acetylated); 0,037 pmol/ml (acetylated)
- Tests per kit:96 tests
Specifications
About this item
This direct cAMP ELISA kit is the most sensitive colorimetric kit for intracellular cAMP quantification. For the quantitative determination of cAMP in cell lysates and tissue from any species. Cited sample types include cell culture supernatant and tissue culture.
- Highly sensitive with an optional acetylation protocol
- High throughput format
- Flexible assay
- Easy to use pre-coated plate and liquid color coded reagents reduce errors
This is a colorimetric competitive immunoassay kit for the quantitative determination of intracellular cAMP in cells or tissues lysed in 0,1 M HCl, which is used to stop endogenous phosphodiesterase activity and stabilize the released cyclic AMP. The 0,1 M HCl treated samples are then analyzed directly in a microtiter plate without extraction, drying and reconstitution. Sensitivity is increased 10-fold by acetylation (reagents included). Absorbance is read at 405 nm. The large signal-to-background ratio offers superior sensitivity compared to competitors. This kit easily assess adenylyl cyclase activation by GPCRs.