Specifically designed for visualizing microscopically nucleoli in living cells.

• Compatible with common live-cell nuclear counterstains (Hoechst 33342, Draq5™, Vybrant® DyeCycle™ Ruby, NUCLEAR-ID® Red dyes)
• High resistance to photobleaching and concentration quenching, ensuring strong, consistent fluorescence signal, even after extended viewing periods
• Validated for utility in live cell imaging applications, demonstrating appropriate response to treatment with well-characterized organelle-perturbation agents
• Stringently manufactured, to control and eliminate non-specific assay artifacts

The kit is compatible with most fluorescence detection systems, including conventional and confocal fluorescence microscopes, as well as High Content Screening (HCS) platforms.

The dye in the kit is resistant to photobleaching, facilitating its use in imaging applications. This nucleolar dye may be used in conjunction with common live-cell nuclear counterstains such as Hoechst 33342, Draq5™, Vybrant® DyeCycle™ Ruby and Enzo’s Nuclear-ID™ Red dye.

A control nucleolus perturbation agent, actinomycin D, is provided for monitoring changes in nucleolar dynamics. Potential applications for this kit include monitoring impaired ribosome biogenesis, inhibition of transcription, cell cycle dynamics and cellular stress, as well as the distribution, trafficking and dynamics of nucleolar proteins, the distribution of viral proteins, and potentially as an aid in identifying cancer cells.