Specifications
- Detector:Two matched side window photomultiplier tubes
- Dispensing volume range:3 - 350 µl for each individual well
- Light source:High energy xenon flashlamp
- Measuring principle:Fluorescence intensity (FI)
Fluorescence Polarisation (FP)
Time-resolved Fluorescence (TRF)
Luminescence (flash and glow) (LUM)
UV-VIS Absorbance spectra (ABS) - Plate types:6 to 1536-well plates
- Resolution:1, 2, 5, 10 nm (selectable)
- Description:Multimode plate reader
- Dispensers:Up to 2 built-in optional reagent injectors; injection at measurement position ( 6 -384 well); up to 4 individual injection events/well; reagent backflushing
- Wavelength bandwidth:220 - 850 nm
- Temperature stability:±0,2 °C
- Absorbance accuracy:<1% at 2 OD
- Dispensing speed:Variable injection speed 100 to 420 µl/s
- Dynamic range:8 Orders (LUM)
8 Orders FI
7 Orders (high end TRF) - Filter type:Excitation and emission filter wheel for 8 filters each
- Interfaces:USB
- Measuring modes:Top and bottom plate reading
- Scan speed:<1 s/well for full spectrum capture
- Sensitivity:< 0.2 fmol/well fluorescein (FI)
<5 mP SD @ 1 nM sodium fluoresein (FP)
<30 amol/well europium (TRF)
<3amol/well europium (high end TRF) ( option)
<20 amol/well ATP (LUM)
<100 amol Alphascreen - Shaking:Linear, orbital and double orbital
- Weight:29 kg
- W×D×H:440×480×310 mm
- Incubation range:Ambient +5...45 °C (60 °C option)
- Measurement time (96-well plate):9 seconds (flying mode)
- Measurement time (384-well plate):16 seconds (flying mode)
- Pk:1
Specifications
About this item
The POLARstar Omega is high speed full spectrum absorbance multidetection microplate reader with simultaneous dual emission detection and fluorescence polarisation. Modes include standard fluorescence, time-resolved fluorescence, fluorescence polarisation (anisotropy), UV/Vis absorbance (OD) measurement of complete spectra or individual wavelengths and high performance luminescence.
- Multi fluorophore detection and full absorbance spectra
- Simultaneous dual emission detection system
- Automatic optic switching for detection mode changes and top/bottom reading
- Two optional 'smart' injectors; injection directly at the measurement position
- Precise temperature control and flexible shaking
The POLARstar Omega offers all the features of the FLUOstar Omega plus a unique simultaneous dual emission detection system, which allows the detection of two emitted wavelengths at the same time. Simultaneous Dual Emission Detection is especially useful for FRET, TR-FRET, BRET and Fluorescence Polarisation assays. Like all readers in the Omega range the POLARstar Omega benefits from the unique UV/Vis spectrometer providing spectral data acquisition quicker than any traditional scanning technology.
The unit can be configured with on-board 'smart' injectors and has a temperature controlled incubation chamber with an extended range to 60 °C option. The wavelength range for standard and TR fluorescence is 240 nm to 740 nm (extended wavelength to 900 nm is available, please enquire for details). For higher throughput the POLARstar Omega can be integrated into most types of robotic systems from a variety of manufacturers or used with a 50-plate stacker with an integrated barcode reader (please ask for details).
The POLARstar Omega is certified by Promega for the Dual Luciferase Reporter (DLR™) gene assay, certified by CisBio Bioassays for their HTRF® assay platform, and has LanthaScreen™ certified plus status. It is also validated from Bellbrook Labs for Transcreener® in 96-well plate format.