Tween® 20 (Polysorbate)

TWEEN 20 is a nonionic detergent widely used in biochemical applications, such as emulsifying agents for the preparation of stable oil-inwater emulsions. It has been used in preextraction of membranes to remove peripheral proteins. TWEEN 20 has been used as a blocking agent for membrane based immunoassays at a typical concentration of 0.05%. TWEEN 20 can be used for lysing mammalian cells at a concentration of 0.05 to 0.5%.

TWEEN 20 is a polyoxyethylene sorbitol ester.

Typical Working Concentration:
Extraction of membrane-bound proteins: 2%; blocking agent for membrane based immunoassays at a typical concentration of 0.05%; lysing mammalian cells at a concentration of 0.05 to 0.5%.

• Research grade