The Ouchterlony Plates have eight antigen wells (8 mm diameter), each surrounded by six antisera wells (3 mm diameter). These plates have been especially designed for work involving antigens present in very low concentrations. The large antigen well allows 75 μl of sample to be transferred at one time. The format is particularly useful for isotyping of tissue culture supernatants of monoclonal antibodies.

The plate contains 1.2% high quality immunodiffusion grade agarose and 3% PEG to enhance the sensitivity of the immunodiffusion assay. The buffer is PBS plus 0,1% sodium azide and 0,01% merthiolate as preservatives.