Specifications

• Source:
  E. coli
• Species:
  Human
• Storage conditions:
  Lyophilized Epidermal Growth Factor 21 Leu although stable at room temperature for weeks, should be stored desiccated below −18 °C. Upon reconstitution EGF 21-Leu should be stored at 4 °C between 2-7 days and for future use below −18 °C. For long term storage it is recommended to add carrier protein (0. 1% HSA or BSA). Please prevent freeze/thaw cycles.
• Biological activity:
  The ED50, calculated by the dose-dependant proliferation of MDCK cells is 10 ng/ml.
• Protein synonyms:
  Urogastrone
• Protein/peptide name:
  EGF 21-Leu
• Purity:
  Greater than 98.0% as determined by RP-HPLC and analysis by SDS-PAGE
• Solubility:
  It is recommended to reconstitute the lyophilized Epidermal Growth Factor 21-Leu in sterile H2O not less than 100ug/ml, which can then be further diluted to other aqueous solutions.
• Sequence:
  The sequence of the first five N-terminal amino acids was determined and was found to be Asn-Ser-Asp-Ser-Glu.
• Formulation:
  The protein was lyophilized from concentrated (1mg/ml) solution with no additives.
• Tested applications:
  SDS-PAGE , HPLC , FuncS