Thermo Scientific™ SMART Digest™ ImmunoAffinity (IA) Kits are designed to provide fast, simple, highly reproducible and sensitive immunocapture and protein digestion in a single well. These kits remove the challenges often associated with the process of biomarker quantitation, by providing a simple and easy capture-digestion workflow that takes only hours instead of overnight.
- Significantly faster than traditional protocols
- Easy to use
- Highly sensitive and reproducible
- Compatible with automation
The SMART Digest™ IA Kits are uniquely designed whereby the immunoaffinity reagents (either streptavidin, protein A or protein G) and heat activated thermally stable trypsin are co-immobilised onto a single bead. Following the binding of a capture reagent to the bead, and enrichment of the target, the enzyme is activated at elevated temperatures for accelerated digestion under protein denaturing conditions. The resulting workflow is as easy as enrich, wash and digest. Magnetic and non-magnetic versions of the beads are available.