West HisProbe kit, SuperSignal™
Catalog # PIER15168
Supplier: Thermo Fisher Scientific
CAS Number:
Specifications
- Pk:1 KIT
- Description:SuperSignal™ West HisProbe Kit, sufficient for 62 mini blots or 5000 cm2 membrane
- Environmentally Preferable:
Specifications
About this item
SuperSignal™ West HisProbe Kit contains HisProbe-HRP conjugate and reagents required for detection of polyhistidine-tagged protein by Western blotting. HisProbe-HRP Conjugate is a nickel (Ni²⁺)-activated derivative of horseradish peroxidase (HRP) that enables direct, IMAC-based detection of His-tagged proteins.
- One-step probe incubation eliminates the need to run a lengthy two-step primary/secondary antibody reaction protocol
- Recognise polyhistidine tags independent of adjacent tags
- Smaller than antibodies and detects his tagged fusion proteins that are often undetectable
The active ligand in HisProbe-HRP is a tridentate chelator that allows Ni²⁺ to be bound in active form for subsequent interaction and detection of target molecules. The active chelator has binding capabilities similar to iminodiacetic acid (IDA) when used for immobilised metal affinity chromatography (IMAC).
Delivery: Kit includes HisProbe-HRP conjugate (2 mg), SuperSignal™ West Pico Luminol/Enhancer solution (250 ml), SuperSignal™ West Pico stable peroxide solution (250 ml), Blocker BSA in TBS, 10X (125 ml), TBS packs (10 packs, each making 500 ml), Surfact-Amps™ 20 detergent solution (6×10 ml).