Pierce™ Horseradish peroxidase (HRP) is purified horseradish peroxidase enzyme for use in activity assays and conjugation to antibodies for ELISA, Western blot and immunohistochemistry applications.

• Specific activity is greater than 300 units/mg
• HRP is smaller (40 kDa) than alkaline phosphatase (AP, 140 kDa)
• Higher specific enzyme activity than both AP and beta-galactosidase (b-Gal)

This purified horseradish peroxidase (HRP) is supplied lyophilised as a salt-free powder for reconstitution and use in protein research methods. The main application for HRP in molecular biology and protein research is as a reporter system for immunoassays and other probe-based assay techniques such as ELISA, Western blotting, EMSA and Southern blotting. The enzyme is usually conjugated to specific secondary antibodies or streptavidin, and its activity is detected with a colour-forming (or light-generating) substrate.