The Protein A IgG Purification Kit is useful for routine purification of antibodies. Samples containing IgG are incubated with immobilised Protein A in a buffer that facilitates binding. After non IgG components are washed from the column, the bound IgG is recovered by elution. The supplied buffers provide for maximum immunoglobulin binding and elution efficiency with the Protein A column.

• Immobilised Protein A is ideal for polyclonal IgG purification from human, rabbit, pig, dog or cat serum
• Agarose resin support is crosslinked 6% beaded agarose (CL-6B), the most popular resin for protein affinity purification methods
• Superior manufacturing method immobilises Protein A by charge-free, leach-resistant covalent bonds, resulting in low non specific binding and enabling multiple uses without decline in yield
• Pre-packed Protein A columns and desalting columns supplied in the kit make IgG purification easy and efficient

Pierce™ Protein A Agarose has a normal load of immobilised Protein A, providing a binding capacity of 12 to 19 mg human IgG/ml resin (approx. 6 to 8 mg mouse IgG/ml resin).