Ficoll® PM400
About this item
Ficoll® PM400 is a synthetic neutral, highly branched hydrophilic polymer of sucrose with an average molecular weight of 400000. It is used to form density gradients for separating and isolating eukaryotic cells, organelles and bacterial cells, as a stabilising agent and as a preparation medium for isolating mononuclear cells. It is useful for separating cells that are sensitive to centrifugation. Ficoll™ PM400 can also be used in other applications, such in defined culture media, nucleic acid hybridisation, electrophoresis, and immunological studies.
- Concentrations of up to 50% (w/v) covering densities of up to 1,2 g/ml can be achieved
- Better osmotic properties than sucrose
- Preserves functional and morphological integrity
- Does not penetrate biological membranes
- Serves as the raw material for preparation of Ficoll-Paque gradients
3 Options Available Below
Product Details & Documents
Ficoll® PM400 is a synthetic neutral, highly branched hydrophilic polymer of sucrose with an average molecular weight of 400000. It is used to form density gradients for separating and isolating eukaryotic cells, organelles and bacterial cells, as a stabilising agent and as a preparation medium for isolating mononuclear cells. It is useful for separating cells that are sensitive to centrifugation. Ficoll™ PM400 can also be used in other applications, such in defined culture media, nucleic acid hybridisation, electrophoresis, and immunological studies.
- Concentrations of up to 50% (w/v) covering densities of up to 1,2 g/ml can be achieved
- Better osmotic properties than sucrose
- Preserves functional and morphological integrity
- Does not penetrate biological membranes
- Serves as the raw material for preparation of Ficoll-Paque gradients
Specifications for Product Family
Specifications
- Catalog No:
- 17-0300-10
- 17-0300-50
- 17-0300-05
- 17-0300-08
- Stability:
- Stable in alkaline and neutral solutions, but are rapidly hydrolyzed in solution at pH 3, especially at elevated temperature
- Stable in alkaline and neutral solutions, but are rapidly hydrolyzed in solution at pH 3, especially at elevated temperature
- Stable in alkaline and neutral solutions, but are rapidly hydrolyzed in solution at pH 3, especially at elevated temperature
- Stable in alkaline and neutral solutions, but are rapidly hydrolyzed in solution at pH 3, especially at elevated temperature
Specifications
Product Family Options
Product Information
- AvailabilityPrice
- 17-0300-10Ficoll™ PM400 (dry powder)Specifications:
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Specifications
Cat. No.17-0300-10StabilityStable in alkaline and neutral solutions, but are rapidly hydrolyzed in solution at pH 3, especially at elevated temperature - 17-0300-50Ficoll™ PM400 (dry powder)Specifications:
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Specifications
Cat. No.17-0300-50StabilityStable in alkaline and neutral solutions, but are rapidly hydrolyzed in solution at pH 3, especially at elevated temperature - 17-0300-05Ficoll™ PM400 (dry powder)Specifications:
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Specifications
Cat. No.17-0300-05StabilityStable in alkaline and neutral solutions, but are rapidly hydrolyzed in solution at pH 3, especially at elevated temperature - 17-0300-08Ficoll™ PM400 (dry powder)Ficoll™ PM400 (dry powder)Discontinued-Specifications:
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Specifications
Cat. No.17-0300-08StabilityStable in alkaline and neutral solutions, but are rapidly hydrolyzed in solution at pH 3, especially at elevated temperature