Size exclusion chromatography columns, Superdex™ 75 10/300 GL and 5/150 GL
: Cytiva
Superdex™ 75 are pre-packed size exclusion chromatography columns for high resolution, small scale, preparative and analytical separations of biomolecules. They have a separation range for molecules with molecular weights between 3000 and 70000 and are suitable for many tagged recombinant proteins. Superdex™ 75 GL has small bed volume, which is useful for a range of applications such as screening solubilisation conditions for membrane proteins, the study of protein-protein interactions and rapid purity check of proteins. These columns are an excellent choice when time and sample/buffer consumption is more important than the highest resolution since less buffer and sample volumes are required.
- High resolution separations of proteins, peptides and other biomolecules according to size
- Ideal for the polishing step in a purification procedure
- Easy and predictable scale up
- For rapid protein homogeneity analysis or purity check use the 5/150 GL short columns