The Biosensis NGFR/p75ECD Rapid™ enzyme-linked immunosorbent assay (ELISA) kit is a sandwich ELISA that allows the quantification of the extracellular domain (ECD) of mouse p75 neurotrophin receptor in less than 4 hours in cell culture supernatants and urine only if used as directed.

This NGFR/p75^ECD ELISA kit employs a recombinant mouse p75^ECD-Fc chimera as standard. While there is a current lack of a true mouse p75^ECD standard, this ELISA kit allows quantification of mouse p75^ECD as p75^ECD–Fc mouse equivalents. Please note that the antibodies used in this ELISA cross-react with human NGFR/p75^ECD.

The purpose of this kit is the in vitro quantitative determination of mouse p75^ECD in samples such as cell culture supernatants and urine. This kit has not been tested for other applications.

This kit consists of a pre-coated mouse monoclonal anti-p75^ECD capture antibody, a goat anti-p75^ECD detection antibody and a horseradish peroxidase (HRP)-conjugated anti-goat antibody. The addition of a substrate (3,3’,5,5’-tetramethylbenzidine, TMB) yields a coloured reaction product which is directly proportional to the concentration of p75^ECD present in samples and protein standards.

Please refer to the kit protocol for specific use instructions for each substrate application.