Glyceraldehyde 3-Phosphate Dehydrogenase (GAPDH) is a metabolic enzyme responsible for catalyzing one step in the glycolytic pathway, the reversible oxidative phosphorylation of glyceraldehyde 3-phosphate. GAPDH may have other roles in the activation of transcription and in the regulation of apoptosis as well as Alzheimer's disease and Huntington's disease. The immunogen used to raise this particular antibody was extensively purified pig GAPDH. This antibody can be used as a loading control for western blotting experiments, allowing comparison between the level of this protein and others in a cell or tissue.

The specificity of this antibody has been confirmed by WB.
Application Information:
Western Blotting (WB) and Immunocytochemistry (IC). A dilution of 1:1,000 is recommended for WB. Human GAPDH has a predicted length of 335 residues and a MW of 36 kDa. A dilution of 1:100 is recommended for IC. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.

Type: Primary
Antigen: Glyceraldehyde 3-Phosphate Dehydrogenase
Clonality: Monoclonal
Clone: 1D4
Conjugation: Unconjugated
Epitope:
Host: Mouse
Isotype: IgM
Reactivity: Bovine, Chicken, Human, Mouse, Pig, Rat