About this item
The RB6-8C5 antibody binds to mouse Ly-6G, commonly known as Gr-1, a member of the Ly-6 superfamily of GPI-anchored cell surface proteins with roles in cell signaling and cell adhesion. Gr-1 is differentially expressed during development and maturation of cells in the myeloid lineage and is expression at varying stages and levels on monocytes, macrophages, granulocytes, and peripheral neutrophils.
The RB6-8C5 antibody is typically used in combination with the macrophage labeling antibody M1/70 (Anti-CD11b) for phenotypic analysis of monocytes, macrophages and granulocytes. Note: The RB6-8C5 antibody has been reported to cross-react with Ly-6C on cells expressing this antigen (Fleming et al. 1993. J. Immunol. 151:2399-2408 and Sasmono et al. 2007. J. Leukoc. Biol. 82: 111-123) and has been cited in the literature for identification of Ly-6G/Ly-6C. Other reports suggest that this antibody is specific for Ly-6G, without cross-reactivity for Ly-6C (Nagendra S. and Schlueter AJ. 2003. Cytometry A, 58(2): 195-200.
Type: Primary
Antigen: Ly-6G (Gr-1)
Clonality:
Clone: RB6-8C5
Conjugation: redFluor™ 710
Epitope:
Host: Rat
Isotype: IgG2b
Reactivity: Mouse
Specifications
- Catalog No:
- 80-5931-U100
- 80-5931-U025
- Antigen symbol:
- LY6C1
- LY6C1
- Antigen name:
- Lymphocyte Antigen 6 Complex, locus C1
- Lymphocyte Antigen 6 Complex, locus C1
- Antigen synonyms:
- Ly6c|AA959465|Ly-6C|Ly-6C1|AA682074
- Ly6c|AA959465|Ly-6C|Ly-6C1|AA682074
- Antibody type:
- Primary
- Primary
- Conjugation:
- redFluor® 710
- redFluor® 710
- Clone:
- RB6-8C5
- RB6-8C5
- Reactivity:
- Mouse
- Mouse
- Host:
- Rat
- Rat
- Gene ID:
- 17067
- 17067
- Isotype:
- IgG2b
- IgG2b
- Flow cytometry:
- Yes
- Yes
- Neutralisation:
- Yes
- Yes
- Storage temperature:
- Store undiluted at 2-8 °C, protect from light, do not freeze.
- Store undiluted at 2-8 °C, protect from light, do not freeze.
- Tested applications:
- Flow cytometry
- Flow cytometry